![]() ![]() ![]() Studies involving multiple imaging sessions involve survival surgeries, which incur additional considerations for anesthesia, surgical preparation, and pain management, as described below. Since the animal is euthanized at the end of a single-session imaging study, the surgery is considered a non-survival surgery. Intravital microscopy studies may consist of a single imaging session or of a sequence of multiple imaging sessions, as for longitudinal studies in which an animal is repeatedly imaged over periods of days or weeks. IACUCs follow federal regulations as they pertain to the Animal Welfare Act, National Research Council’s Guide for the Care and Use of Laboratory Animals, and the Public Health Services Policy on Humane Care and Use of Laboratory Animals. In the United States, each research institution must have an IACUC that reviews proposals for research, teaching or testing activities involving vertebrate animals, and approval is required prior to initiating any such activities. Prior to any animal studies, appropriate institutional approval must be obtained. These methods were designed in accordance with guidelines provided by Indiana University’s Institutional Animal Care and Use Committee (IACUC). The methods described here were optimized to provide access to abdominal organs such that the tissues are sufficiently immobilized to support high-resolution imaging, while preserving the animal and organ physiology. Here, I describe methods of anesthesia, surgical techniques, and methods to maintain and monitor animal physiology that we have developed for intravital microscopy of the kidney, liver, spleen and pancreas of rats and mice. ![]() However, meaningful results can only be obtained from animals whose physiology is preserved during microscopy. Therefore, models of disease specific to these organs may be studied in the rat and mouse, potential treatment modalities may be tested thereafter, and, the application of such treatment modalities may potentially be transferred to humans. The utilization of the rat and mouse models can be advantageous in that organ structure and function in these species mimics humans. īavaria Fiction is making a push into international TV and is producing the “Das Boot” series, as well as Deutsche Telekom comedy “Germanized.Intravital microscopy has afforded researchers the ability to better understand cellular and subcellular processes within multiple organs in the body. “The show gathers some incredible international talents to tell a unique story that should entertain large audiences worldwide,” said Frédérik Rangé, director of acquisitions at Lagardère Studios Distribution. Lagardère Studios Distribution has taken the international rights and will sell the series. “The audience will feel the desolation, the blistering wind, the taste of blood, and the cold, but also the love, warmth, and commitment created by our strong main characters.” The producers say that the series will eschew the typical dark and brooding Nordic noir tropes while remaining in the crime genre. “I want ‘Arctic Circle’ to be a physical experience,” said director Hannu Salonen (“Shades of Guilt”). Finnish pubcaster YLE has picked up the free-to-air rights. Yellow Film & TV and Bavaria Fiction are making the series for Finnish streaming service Elisa Viihde, which will launch the series next year. ![]()
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